Table 1.
Crystal | Thio intermediate | DAD TS structure |
---|---|---|
Diffraction Data | ||
Space group | P212121 | P212121 |
Unit cell (Å) | 46.5, 60.4, 107 | 45.8, 59.7,106 |
Resolution | 2.5 Å | 1.9 Å |
Measured reflections | 40,888 | 60,754 |
Unique reflections | 10,834 | 22,339 |
Completeness (%) | 99.5 (96.0) | 99.6 (96.4) |
Avg I/Sigma | 14.3 | 15.2 |
Multiplicity | 2.1 | 3.2 |
Rmerge | 0.11 (.45) | 0.10 (.37) |
Refinement | ||
Resolution range (Å) | 2.5 | 50–1.9 |
Reflections | 10834 | 22339 |
Working | 10287 | 21169 |
Test (5%) | 547 | 1170 |
Total atoms | 1982 | 2039 |
Protein | 1845 | 1770 |
peptide | 68 | 92 |
S-alkylamidate | 40 | ------- |
Water molecules | 87 | 145 |
R (%) | 22.4% | 19.7% |
Rfree (%) | 24.5% | 22.9% |
rmsd | ||
Bond lengths (Å) | 0.015 | .016 |
Bond angles (°) | 1.6 | 1.4 |