Fig. 6.

Lymphocyte infiltration and astrocytosis in PD2 mice. (A) CD3, CD8 and CD68 levels in the brains of PD2 mice. Immunoslot blots were used to determine the reactivity of CD3, CD8 and CD68, using anti-CD3, anti-CD8 and anti-CD68 antibodies, in brain homogenates from SJL mice with acute EAE, and from 2-, 4-, 6- and 9-month-old normal and heterozygous PD2 mice. The loading control for each individual was reactivity of the anti-histone H3 antibody. (B) Immunoslot blot analysis of the amount of GFAP in normal and heterozygous PD2 mice, as measured by GFAP immunoreactivity in whole brain homogenates using anti-GFAP antibody. The graph shows the amount of GFAP in PD2 mice relative to their normal littermates, as determined from the ratio of GFAP to the loading standard mouse actin. At each age, the ratios of GFAP to actin pixel densities from the PD2 mice were normalized to those of their normal littermates.