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. Author manuscript; available in PMC: 2009 Nov 15.
Published in final edited form as: Dev Biol. 2008 Sep 9;323(2):207–215. doi: 10.1016/j.ydbio.2008.08.029

Figure 5. UNC-3 can form homodimers.

Figure 5

HEK293 cells were transfected with the indicated constructs, immunoprecipitations were performed using the indicated antisera, proteins were resolved using SDS-PAGE, and UNC-3 was detected on western blots with UNC-3 antisera that distinguished full length and truncated UNC-3. (A) mycUNC-3ΔC-term, a myc-epitope-tagged construct that encodes an UNC-3 protein lacking the C-terminal 31 amino acids to which the UNC-3 antibody (αUNC-3 C-term) binds; the protein generated by this construct is not recognized by the αUNC-3 C-term antiserum, which was raised against this C-terminal peptide (2). Filled arrowhead indicates full-length UNC-3. The asterisk indicates an irrelevant band detected by the UNC-3 antiserum running slightly below UNC-3. (B) Filled arrowhead indicates mycUNC-3ΔC-term.