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. Author manuscript; available in PMC: 2009 Nov 15.
Published in final edited form as: Dev Biol. 2008 Sep 9;323(2):207–215. doi: 10.1016/j.ydbio.2008.08.029

Figure 6. Coimmunoprecipitation of PAG-3 and UNC-3.

Figure 6

HEK293 cells were transfected with the indicated constructs, myc-epitope-tagged PAG-3 was immunoprecipitated with anti-myc antiserum, proteins were resolved using SDS-PAGE, and UNC-3 was detected on a western blot with UNC-3 antiserum (αUNC-3). Filled arrowheads indicate UNC-3 protein. The asterisk indicates an irrelevant band detected by the UNC-3 antiserum running slightly below UNC-3.