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TAR RNA construct and modifications used in this study. (A) Wild-type TAR RNA construct from HIV-1 containing the UCU bulge at positions 23–25 and the conserved base pairs A27–C38, C26–C39, A22–U40, and G21–C41 are shown in italics; (B) UUU-TAR, where C24 has been changed to U24; (C) CCU-TAR, where U23 changed to C23; and (D) CCC-TAR, where U23 and U25 have been changed to cytosine.
