FIGURE 6.

miRNA-mediated repression of 3′ UTRs fused to luciferase reporter genes. Luciferase activity from HEK293 cells cotransfected with each reporter/control psiCHECK construct and 2′-O-methyl oligoribonucleotides antisense to 12 HEK293 miRNAs (red bars) was normalized to that from cotransfection of each reporter construct with 2′-O-methyl oligoribonucleotide antisense to Drosophila miRNA bantam (blue bars). Transfection of parental psiCHECK vector without inserted 3′ UTR (psiCHECK) and 3′ UTR with two artificial binding sites each for miR-16 and miR-196b (psiCHECK T) are indicated. Error bars represent standard deviation of experiment performed with six replicates. Normalized Renilla luciferase (RL) versus firefly luciferase (FL) activities are indicated.