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. 2003 Nov 15;112(10):1571–1580. doi: 10.1172/JCI18986

Figure 2.

Figure 2

mAb 146B7 inhibits protection of apoptosis by IL-15. Human PBMCs were incubated with IL-15 (5 ng/ml) in culture medium alone (filled squares) or in combination with 10 μg/ml mAb 146B7 (open circles) or with 10 μg/ml isotype control antibody (filled triangles) for 5 days. Apoptosis was measured using annexin V conjugated with FITC and PI. Live (a and d), early (b and e), and late (c and f) apoptotic cells were defined by annexin V-FITCnegPIneg, annexin V-FITCposPIneg, and annexin V-FITCposPIpos staining by flow cytometry, respectively. Representative data of three individual experiments with six different PBMC donors are shown. (a–c) mAb 146B7 inhibits IL-15–induced survival of monocytes. (d–f) No significant differences in comparison were found in the fraction of live and apoptotic T cells. *P < 0.05, mAb 146B7 versus no antibody treatment. **P < 0.005, mAb 146B7 versus no antibody treatment, and mAb 146B7 versus anti-KLH.