Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Human insulin stimulated extracellular signal-regulated kinase (ERK, A) and Akt/protein kinase B (PKB, B) phosphorylation in the A. stephensi midgut. Midgut proteins prepared from A. stephensi at 30 min after feeding on artificial bloodmeal supplemented with human insulin were probed with anti-phospho-ERK antisera (p-Erk, 1:10 000; A) or with anti-phospho-Akt antibody (p-Akt, 1:1000; B). Anti-total ERK (ERK) or anti-total Akt/PKB (Akt) antibody was used to assess loading.
