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IFNγ release assay of MHC restriction of CD8+ clone 663·14: Dot plot FACS profiles of bovine endothelial cells stained with class II MHC-specific mAb IL-A21 showing a representative analysis of IFNγ release by a CD8+ clone. From left to right, cells cultured with supernatant from clone 663·14 incubated with an MHC-mismatch target, the autologous target, an A18 homozygous target, and an A14 homozygous target. All targets were infected with the T. annulata Ankara C9 stock.
