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. 2008 Dec 1;105(49):19474–19479. doi: 10.1073/pnas.0810305105

Fig. 5.

Fig. 5.

L. acidophilus NCFM SlpA mediates binding to DC-SIGN on iDCs. (A and B) Microscopic analysis of DCs interacting with fluorescently labeled L. acidophilus NCFM (A) and SlpA-knockout mutant, NCK1377-CI (B). (Scale bars, 5 μm.) (C and D) FACScan analysis of L. acidophilus NCFM or NCK1377-CI binding to iDCs when iDCs (5 × 104 cells) were treated with 5 × 106 FITC-labeled L. acidophilus NCFM (SlpA+) (C) or SlpA-knockout NCK1377-CI (SlpB+) (D) for 45 min at 37 °C. (E and F) Blocking antibodies to DC-SIGN (AZN-D1, c = 20 μg mL−1) were used when iDCs were treated with L. acidophilus NCFM (E) or NCK1377-CI (F). A representative experiment of 4 is shown.