Table 1.

Summary of motility and load-dependent kinetic parameters measured at saturating [ATP]

Kinesin	Load						No load
	Stall force,*Fs (pN)	Blotzmann			Fisher 2-state		v(0)*, nm/s	L*, μm
		vmax,† nm/s	A†	δ,† nm	d0, nm	d1, nm
WT	4.96 ± 0.05	493.7 ± 26.4	0.0043 ± 0.0050	5.53 ± 1.04	4.4	3.8	581.1 ± 38.8	1.104 ± 0.215
2G	3.02 ± 0.03	535.2 ± 27.8	0.0137 ± 0.0101	7.15 ± 1.10	1.1	7.1	608.2 ± 22.5	1.740 ± 0.209
DEL	1.37 ± 0.04‡	482.1 ± 33.5	0.0357 ± 0.0202	11.28 ± 1.24	0.4	7.8	254.8 ± 27.2	0.342 ± 0.088

Number of measurements: n_loaded = 373, 487, 117; n_unloaded = 34, 75, 32 (WT, 2G, DEL).

*Mean ± SEM.

^†Mean ± 95% confidence interval.

^‡Potentially biased toward a higher mean value on account of the unknown number of stalling events below the minimum detection threshold of 0.7 pN.