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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: J Neurovirol. 2008 Oct 18;14(5):437–447. doi: 10.1080/13550280802356845

Figure 4. IFN-gamma and cytokine effects on NSCs.

Figure 4

NSCs were treated with the indicated cytokine for 4 d. MTT uptake assay was performed on d 4 post-treatment to determine the effects of cytokine treatment on NSC proliferation. (A) Dose-dependent decrease in NSC growth was observed following IFN-gamma treatment (* p=0.014; ** p<0.01). (B) IL-1beta (IL-1) and TNF-alpha (TNF) did not alter NSC proliferation. Data are presented as percent of control (untreated cells) from 3 experiments at 4 d post cytokine treatment (± SD). (C) Similar dose dependent decrease in 3H-thymidine uptake by IFN-γ treated NSC was observed. Data presented as % of untreated control were obtained from 2 separate experiments with duplicate wells for each treatment. (D) This decrease was evident as early as 24 h post treatment with the cytokine as demonstrated by the kinetics of cell proliferation in IFN-treated (open triangles) and untreated (closed circle) cultures.