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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: Cancer Chemother Pharmacol. 2008 Apr 1;63(2):351–362. doi: 10.1007/s00280-008-0745-3

Fig. 7.

Fig. 7

Inhibition of activation of caspases 3 and 9 by selenite by overexpression of MnSOD in LNCaP cells. a Chemiluminescence analysis of caspase 9 activity. Cells were transduced with 50 MOI AdEmpty or AdMnSOD for 48 h and then treated with 2.5 μM selenite for 18 h. Twenty micrograms of total protein from LNCaP cells with or without AdEmpty or AdMnSOD transduction were mixed with the caspase-9 analysis reagent in 96-well plates. Chemiluminescence was measured using a luminometer. Each bar represents the average of three wells. Data are presented as means ± SD of three independent experiments. * P < 0.05 compared with cells without selenite treatment and AdMnSOD with selenite treatment. b Fluorescence analysis of caspase 3 activity. Cells were transduced with 50 MOI AdEmpty or AdMnSOD for 48 h and then treated with 2.5 μM selenite for 18 h. Fluorescence was measured using a fluorescence microplate reader. Data are presented as means ± SD of three independent experiments. * P < 0.05 compared with AdEmpty without selenite. ** P < 0.05 compared with AdEmpty without selenite and AdMnSOD with selenite.