Figure 6. Single-molecule photobleaching of mKikGR.

(A) Schematic illustration of experimental configuration. The excitation power of the 405 nm and 568 nm lights were set at 6.3 and 130 W cm⁻², respectively. (B) Time course of the fluorescence intensity of the red mKikGR embedded in polyacrylamide gel (100 pM, pH 7.0). The red and blue lines show a single- and double-exponential fit. (C) Excitation power dependence of the photobleaching rates of the red mKikGR.