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. Author manuscript; available in PMC: 2008 Dec 2.
Published in final edited form as: Biochemistry. 2007 Aug 9;46(35):10192–10201. doi: 10.1021/bi7003476

FIGURE 2.

FIGURE 2

Catalytic titrations using P450 2E1 as the titrant. (A) Reaction rates were plotted as a function of P450 2E1 concentration. The reported values reflect the results from average of 2–4 experiments including the standard deviation of the mean. The concentration of CPR-K56Q in each titration was 15, 30, and 60 nM, as indicated by the white to black filling of the respective squares. The fitted line reflects the fit of each data set to a binding quadratic equation (Equation 1). (B) The maximal rates from each titration were plotted as a function of the concentration of the limiting complex partner, CPR-K56Q, and then fit to a linear regression to determine kcat.