Effects of 10−8 M captopril (CPT,
(a) and (b)), zofenoprilat (ZOF,
(a) and (c)), lisinopril (LIS,
(a) and (d)), enalaprilat (ENA,
(a) and (e)) and bradykinin (BK,
(a) and (f)) on endothelin-1 secretion (expressed as % of control) by vascular
endothelial cells derived from umbilical cord vein after 8 hours of incubation
both alone and in the presence of either Des-Arg9-[Leu8]-BK,
that is, a bradykinin B1 receptor antagonist (B1ra, 10−6 M), or D-Arg-[Hyp3, Thi5,8, D-phe7]-BK,
that is, a bradykinin B2 receptor antagonist (B2ra, 10−6 M), or the NO synthase competitive inhibitor
Nω-nitro-L-arginine methyl ester (L-NA, 3 × 10−3 M). (A) P < .0008
or less versus CPT,
ZOF, LIS, ENA, and BK; (B) P < .04 or less versus ZOF; (C) P < .0005
or less versus CPT+B1ra; (D) P < .0003 or less versus ZOF+B1ra,
ZOF+B2ra, and ZOF+L-NA; (E) P < .002 versus ZOF+B1ra; (F) P < .0001
versus LIS+B1ra; (G) P < .0002 or less versus ENA+B1ra; (H) P < .0001
versus BK+B1ra.