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. 2008 Dec;19(12):5104–5115. doi: 10.1091/mbc.E08-02-0224

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© 2008 by The American Society for Cell Biology

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Figure 3. — Depolymerization of cytoplasmic microtubules does not result in Klp5 nuclear entry during interphase. (A) Experimental plan. At time 0, cultures were split into two parts and MBC (50 μg/ml) was added in one of them. After 15 min, LMB (50 ng/ml) was further added in both cultures. Time points (T) refer to minutes. (B) Line profiles of fluorescence intensity showing nuclear accumulation of Klp5-GFP during interphase 60 min after LMB addition (T75 of experiment plan). Top graph, LMB; bottom graph, MBC + LMB. n = 5.