Table 1.
Strain list
| Strain | Genotype | Derivation |
|---|---|---|
| 513 | h− | Lab stock |
| AR017 | h−klp5::ura4+ | This study |
| AR065 | h−klp5+-GFP-kan pREP1(mRFP-atb2+) | This study |
| AR066 | h−klp5+-GFP-kan klp6::ura4+pREP1(mRFP-atb2+) | This study |
| AR067 | h+ade6–216 klp5::ura4+klp6+-GFP-kanR pREP1(mRFP-atb2+) | This study |
| AR085 | h−klp6+-GFP-kan pREP1(mRFP-atb2+) | This study |
| AR214 | h−klp5::ura4+klp6::hph pREP41(GFP-klp5+) | This study |
| AR216 | h−klp5::ura4+klp6::hph pREP41(GFP-klp5-NLSmut) | This study |
| AR217 | h−klp5::ura4+klp6::hph pREP41(GFP-klp6-NLSmut) | This study |
| AR218 | h−klp5::ura4+klp6::hph pREP41(GFP-klp6+) | This study |
| AR231 | h+klp5::nat klp6::ura4+ | This study |
| AR245 | h−his7 klp5-NLSmut | This study |
| AR247 | h−his2/7 klp6-NLSmut | This study |
| AR285 | h−kan-nmtP3-GFP-atb2+klp5::ura4+klp6::hph | This study |
| AR287 | h−kan-nmtP3-GFP-atb2+klp5::ura4+ | This study |
| AR289 | h−kan-nmtP3-GFP-atb2+klp6::ura4+ | This study |
| AR374 | h+his2 klp6N(1–410)::ura4+::klp5N(1–404) | This study |
| AR399 | h−klp6N(1–410)::ura4+::klp5N(1–404)-GFP-nat | This study |
| AR402 | h−klp5N(1–404)::ura4+::klp6N( 1–410) | This study |
| AR445 | h+his2 klp5N(1–404)::ura4+::klp6(1–403)-GFP-nat | This study |
| AR449 | h? kan-nmtP3-GFP-atb2+klp6N(1–410)::ura4+::klp5N(1–404) | This study |
| AR450 | h−kan-nmtP3-GFP-atb2+klp5N(1–404)::ura4+::klp6N(1–403) | This study |
| AR614 | h−klp5+-GFP-kan aur1-mCherry-atb2+ | This study |
| AR615 | h−klp5+-GFP-kan klp6::ura4+aur1-mCherry-atb2+ | This study |
| AR616 | h−ade6–216 klp6+-GFP-kan aur1-mCherry-atb2+ | This study |
| AR617 | h+his7 klp5::ura4+klp6+-GFP-kan aur1-mCherry-atb2+ | This study |
| AR640 | h+his2 klp5::ura4+klp6N(1–410)::ura4+::klp5N(1–404)-GFP-nat aur1-mCherry-atb2+pREP41(GFP-klp5+) | This study |
| AR644 | h−his7 klp5::ura4+aur1-mCherry-atb2+pREP41(GFP-klp5+) | This study |
| AR645 | h−his7 klp5::ura4+aur1-mCherry-atb2+pREP41(GFP-klp5-NLSmut) | This study |
| AR646 | h+his2 klp6::ura4+aur1-mCherry-atb2+pREP41(GFP-klp6+) | This study |
| AR647 | h+his2 klp6::ura4+aur1-mCherry-atb2+pREP41(GFP-klp6-NLSmut) | This study |
| AR648 | h+his2 klp5::ura4+klp6-NLSmut aur1-mCherry-atb2+pREP41(GFP-klp5+) | This study |
| AR650 | h−his7 klp5-NLSmut klp6-hph aur1-mCherry-atb2+pREP41(GFP-klp6+) | This study |
| NK28–5A | h−his2/7 klp6::ura4+ | Lab stock |
| MA201 | h−kan-nmtP3-GFP-atb2+ | Lab stock |
All strains listed in this table contain leu1–32 ura4-D18.