Table 2.
Dynamisms of cytoplasmic microtubules in various klp5/6 mutants
| Strain | Growth rate (μm/min) | Shrinkage rate (μm/min) | Catastrophe frequency (events/min) | Rescue frequency (events/min) | Dwell timea (min) | Dynamicityb (dimers/s) | Total observation time (s) |
|---|---|---|---|---|---|---|---|
| Wt | 2.93 ± 0.64 (n = 18) | 7.86 ± 2.27 (n = 33) | 0.44 | 0.40 | 1.26 ± 0.76 (n = 14) | 92.1 | 4620 |
| Δklp5 | 2.55 ± 0.58 (n = 25) | 8.79 ± 3.18 (n = 38) | 0.39 | 0.36 | 1.15 ± 1.00 (n = 19) | 85.9 | 5810 |
| Δklp6 | 2.92 ± 0.63 (n = 14) | 7.43 ± 2.57 (n = 32) | 0.41 | 0.34 | 1.91 ± 1.27 (n = 11) | 80.9 | 4740 |
| Δklp5 Δklp6 | 2.37 ± 0.50 (n = 11) | 7.60 ± 2.77 (n = 30) | 0.38 | 0.35 | 1.64 ± 1.60 (n = 22) | 80.3 | 4800 |
| klp5N + klp5N | 2.64 ± 0.53 (n = 17) | 5.84 ± 1.91 (n = 30) | 0.30 | 0.27 | 2.14 ± 1.38 (n = 14) | 71.1 | 6000 |
| klp6N + klp6N | 2.68 ± 0.74 (n = 20) | 8.18 ± 2.31 (n = 36) | 0.36 | 0.35 | 1.65 ± 1.29 (n = 19) | 80.9 | 6000 |
a Duration of contact time (minutes) between microtubule end and the cell tip before catastrophe. In our measurements, microtubules in all the strains examined continue to grow at the cell end.
b Mean rate of total tubulin exchange calculated using a conversion factor of 1 μm of microtubule length = 1690 tubulin dimers.