Figure 5.

Deletion of RTG2 suppresses defective colony formation in crd1Δ at elevated temperature. (A) Yeast cells were grown in YPD media to the early stationary phase. Cells were seeded on YPD plates and incubated at 37°C for 3–5 d. (B–E) Total RNA was extracted from cells grown to the mid-log phase in YPD or YPDS at 37°C, and mRNA levels were determined by quantitative real-time PCR analysis. Expression was normalized to the mRNA levels of the internal control ACT1. Error bars represent the range of the three independent experiments.