Figure 8.

The crd1Δcnb1Δ mutant exhibits defective colony formation at 35°C. (A–D) Total RNA was extracted from cells grown to the mid-log phase in YPD at 37°C. mRNA levels were determined by quantitative real-time PCR. Expression was normalized to the mRNA levels of the internal control ACT1. Error bars represent the range of the three independent experiments. (E) Yeast cells were grown in YPD media to the early stationary phase. Cells were seeded on YPD plates and incubated at the indicated temperature for 3–5 d.