Figure 1.

Presence of giantin in the homogenate, nuclei, Barnes washing nuclei and nuclear microdomains. The different fractions were prepared as reported in Materials and Methods. The amount correspondent to 30 μg proteins were loaded onto SDS-PAGE electrophoresis in 8% polyacrylamide slab gel. Immunoblot of proteins were probed with anti-giantin (apparent molecular weight 367 kDa) antibodies and visualized by ECL. C, positive control, H35 hepatoma cells; H, homogenate; N, nuclei; N^a, nuclei after Barnes treatment; NMd, nuclear microdomains.