Figure 3.

CNAPTaz is physiologically relevant. (A and B) Steady-state ³²P labeling and analyses were performed as described in Figure 2. In A, the relative abundance of each phospholipid is expressed as a % of the total phospholipid in each strain (mean ± SEM, n = 3–5). Statistical significance (p = 0.05) was determined by one-way ANOVA, with Holm-Sidak pairwise comparisons. In B, the relative abundance of MLCL and CL is expressed as a % of the total phospholipid in each strain (mean ± SEM, n = 3–10) (C) Digitonin extracts from Δtaz1[CNAPTaz] and Δtaz1Δcrd1[CNAPTaz] mitochondria were resolved by 2D BN/SDS-PAGE, and Taz1p complexes were detected by immunoblot. n = 3.