Figure 5.

(A) 3T3 fibroblasts transfected with NM II-A siRNA show a 70% reduction in NM II-A protein levels compared with cells treated with nontargeted siRNA. NM II-A knockdown cells exhibit reduced numbers of bound collagen beads compared with control cells (p < 0.05 after 20 min; 25 cells per time point). (B) Cells transfected with siRNA for NM II-A do not show Rap1 localization to collagen beads (compare b and e). (C) Knockdown of Rap1 reduces collagen-bead binding, but NM II-A still localizes to collagen beads (compare c and f).