Figure 9.

Atg14ΔCC mutant cannot rescue the autophagy-defective phenotype of Atg14-knockdown cells. (A and B) HeLa cells were treated with the indicated siRNA. After 2 d, the cells were transiently transfected with an empty vector, HA-Atg14* (asterisk [*] indicates the siRNA-resistant version) or HA-Atg14ΔCC* plasmid together with the same siRNAs again, and then they were cultured for an additional 2 d. The cells were again transfected with the same expression vectors and cultured for another 1 d. Then, the cells were incubated in starvation medium for 2 h. Total lysates were prepared and p62 expression level (A) and LC3 turnover (B) were determined.