Figure 9.

Effect of ram1 mutation on Ydj1-GR interaction and GR activity. (A) Isogenic WT (PJ43-2B) and ram1 strains (JJ217 and JJ218) were transformed with Flag-GR or a control plasmid. Flag-GR complexes were isolated and analyzed as described for Figure 6 except that cells were grown at 25°C. (B) Strain JJ160 (ydj1 RAM1) expressing WT YDJ1 or ydj1-C406S and strain JJ264 (ydj1 ram1) expressing WT were transformed with plasmids expressing GR and corresponding GRE-lacZ reporter construct. β-Galactosidase activity was assessed as in Figure 8, except that cells were grown at 25°C.