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. 2008 Dec;19(12):5559–5578. doi: 10.1091/mbc.E08-05-0455

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© 2008 by The American Society for Cell Biology

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Figure 1. — Cbk1 kinase inhibition interferes with bud emergence and induces a Swe1-dependent cell cycle delay. (A) Cbk1 inhibition delays bud emergence. cbk1-as (FLY2084) and wild-type cells (BY4741) were released from G1 arrest and placed into fresh medium with 0 μM (DMSO), 5 μM, or 10 μM 1NA-PP1. The percentage of budded cells was plotted over time (n > 200 cells). (B) Cbk1 inhibition induces a Swe1-dependent G2 arrest. cbk1-as and cbk1-as swe1Δ (FLY2236) cells were released from G0 into medium ± 5 uM 1NA-PP1, and at designated time points samples were processed for FACS to reveal the relative DNA content in the cell population. Four hours after release from G0 block, 0 and 11% of mock and drug treated cbk1-as swe1Δ cells contained two nuclei. T0 is time of 1NA-PP1 addition. Note the modest delay in S phase initiation. See Supplemental Figure S3 for data from a similar experiment. (C) Cbk1 inhibition induces a modest Ssd1-independent delay in bud emergence. cbk1-as ssd1-d cells (FLY2204) were synchronized in G1 and released into medium ± 5 μM 1NA-PP1. The percentage of buds was scored over time (n > 200). Similar phenotypes were observed with cbk1Δ ssd1Δ cells (data not shown).