Figure 12.

Cbk1 kinase and Golgi mannosyltransferases are functionally linked. (A) The conditional lethality of cbk1-8 mutants is suppressed by mannosyltransferase overexpression. Ten-fold dilution series of cbk1-8 cells containing empty vector (pGP654) or dosage suppressor plasmids were spotted onto YPD plates and incubated at the designated temperatures. Cells were also spotted onto YPD plates containing 50 μg/ml hygromycin B and incubated at 22°C. The suppressor plasmids are detailed in Supplemental Table S1. (B) Och1 mannosyltransferase localization is aberrant in cbk1-8 cells. Och1-GFP was monitored in wild type and cbk1-8 cells (JLY284, FLY2922). Cells were grown at 22°C, and, where designated, transferred to 35°C for 1 h. Each image was obtained from a single optical plane.