Figure 3.

Filamin B colocalizes with Rac1 in membrane ruffles. (A) HeLa cells incubated with or without IFNα for 30 min were stained with anti-filamin B or anti-Rac1 antibody or phalloidin. The bars indicate 10 μm. (B) Cells transfected with shRNA-1 or shControl were cultured and stained as in A. Bars, 10 μm. (C) Lysates were obtained from the same cells used in B and subjected to immunoblot with anti-filamin B or anti-β-actin antibody. The numerals indicate the quantified band intensities relative to that seen with shControl only and are representative of three independent experiments. (D) The fractions of cells showing colocalization of filamin B with Rac1 in membrane ruffles were quantified. Error bars are ±SEM of total 90 cells from at least three independent experiments.