Skip to main content
. 2008 Dec;19(12):5478–5489. doi: 10.1091/mbc.E08-05-0448

Figure 2.

Figure 2.

Implication of zGRK2/3 in the Hh pathway. (A) Flat-mounted embryos viewed dorsally reveal enriched GRK2/3 message in the tail bud caudally and in the notochord more rostrally (five-somite stage). (B) The expression of shh at the five-somite stage is restricted primarily to the notochord. (C) At 24 hpf, zGRK2/3 is expressed broadly in the embryo but is excluded from the notochord. (D) Expression pattern of smo at 24 hpf is complementary to the zGRK2/3 pattern. (E–P) In situ hybridization of ptc-1 (E, H, K, and N) and the Hh-independent control genes dmrt2 (F, I, L, and O) and wnt11 (G, J, M, and P) in embryos injected with either the 5_mis CT MO (E–G at bud stage, K–M at 24 hpf) or the zGRK2/3 ATG MO (H–J at bud stage, N–P at 24 hpf).