Table 1.

Wortmannin sensitivity of 2x FYVE-EGFP–positive organelles

Cell type and conditions	Time constant (min)	Half-time (min)	Intensity change (%)
PC12 cells
Control (n = 31, 5 cells)	>20	>20	−3 ± 9
Wortmannin (100 nM)
WISO (n = 23, 7 cells)	>20	>20	4 ± 6
WSO (n = 27, 7 cells)	7.2 ± 0.5	4.9 ± 0.3	78.30 ± 4
Wortmannin (1 μM) (n = 43, 6 cells)	1.7 ± 0.4	1.2 ± 0.3	107.3 ± 2
HEK293 cells
Wortmannin (100 nM) (n = 37, 6 cells)	2.7 ± 0.5	1.8 ± 0.3	100.5 ± 2.1

WISO, wortmannin-insensitive organelles; WSO, wortmannin-sensitive organelles.