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. 2008 Dec 8;3(12):e3872. doi: 10.1371/journal.pone.0003872

Figure 3. C-terminal POMs do not bind to linear 25-meric PrP-peptides.

Figure 3

C-terminal-specific POMs were tested for binding on ELISA plates containing immobilized 25-meric PrP-peptides or rmPrP for control. While all antibodies bound strongly to rmPrP23-231, only POM4, 10 – and POM19, not shown – interacted with a 25-mer, spanning amino acids 200–225 of mouse PrP. As positive control, binding of POM11 on the octarepeat region – peptide 65–89 – was used. As expected POM11 did not interact with N-terminally truncated rmPrP121-231, while all other antibodies tested here did. When all peptides were mixed together and immobilized on the well (ALL P-MIX) no binding was observed, probably because of the inefficient amounts of the respective peptides. BLK: Blank.