Table 3.

Deletion phenotypes predicted differently under raffinose conditions by iLL672 builds with and without α-galactosidase activity

Gene	Experimental phenotype of deletion under YPRaff	Predicted phenotype of deletion mutant made by iLL672 build with α-galctosidase	Predicted phenotype of deletion mutant made by iLL672 build without α-galctosidase	Reason for incorrect prediction made by iLL672 build with α-galactosidase activity
Gal1	Viable	Unviable	Viable	Galactose is produced when α-galactosidic bond in raffinose is cleaved. Therefore, in build with α-galactosidase, Gal genes are required to metabolize galactose produced during raffinose catabolism
Gal7	Viable	Unviable	Viable	See explanation for Gal1
Gal10	Viable	Unviable	Viable	See explanation for Gal1
YBR184W	Viable	Unviable	Viable	YBR184W is the putative α-galactosidase and is essential by definition of the model build
Suc2	Unviable	Viable	Unviable	The galactose produced when the α-galactosidic bond is cleaved can be used as the primary carbon source, with the remaining sucrose unit being excreted
Pgi1	Unviable	Viable	Unviable	Pgi1 catalyzes the reversible conversion of glucose-6-phosphate to fructose-6-phosphate in glycolysis and gluconeogenesis. The pentose phosphate pathway can be used to bypass the deletion of this gene to maintain glycolytic function Without the α-galactosidase only the fructose unit of raffinose is usable, making Pgi1 essential for its role in gluconeogenesis