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4-Hydroxynonenal (HNE) induced Fas/CD95 and p53 protein expression and reduced Akt phosphorylation. Chondrocytes were incubated with 30 μM HNE for increasing incubation times. Total cell lysates or nuclear extracts (~20 μg) were subjected to Western analysis (n = 8) using antibodies anti-Fas/CD95, anti-p53, anti-phospho Akt, and anti-total Akt.
