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. 2008 Sep 30;10(5):R119. doi: 10.1186/ar2523

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Copyright © 2008 Kidd et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Characterisation of antibody responses in collagen-induced arthritis (CIA) and experimental autoimmune encephalomyelitis (EAE) using myelin and synovial antigen arrays. (a, b) Synovial and (d, e) myelin arrays were produced by printing synovial and myelin peptides and proteins in ordered arrays on the surface of microscope slides. The arrays were probed with 1:150 dilutions of (a, d) normal, (b) CIA and (e) EAE sera followed by a cyanine 3 labelled anti-mouse IgG/M secondary antibody. Scanned images of the slides are presented in false colour representation. Yellow features represent marker spots to orient the array. Green features represent serum antibody reactivity. Reactive features are highlighted and quantitative values are presented in panels c and f.