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Immunoblot analysis identifies multiple citrullinated polypeptides in tissue lysates from collagen-induced arthritis (CIA) and experimental autoimmune encephalomyelitis (EAE). Lysates were generated from (a) joint tissue harvested from naïve and CIA mice, as well as from (b) brain tissue harvested from naïve and EAE mice. Tissue lysates were separated by SDS-PAGE and subject to immunoblotting with anti-modified citrulline antibodies.
