Figure 6.

Knockdown of α and βCaMKII eliminates the formation of pCaMKII puncta. αCaMKII (A) and βCaMKII (B) mRNA levels in SCG neurons assessed by quantitative RT-PCR. Some cultures infected with lentiviruses expressing GFP alone, shRNAs against α and βCaMKII, or nonsilencing control shRNAs. Data normalized against three housekeeping genes and plotted relative to the GFP only (empty vector) control (see Materials and methods). n = 3–4 experiments done in triplicate. (C) SCG neurons infected as in A and B and stained with an antibody against βCaMKII; nuclei were counterstained with DAPI. Bar, 50 μm. (D) pCaMKII puncta weight from cells infected with α and βCaMKII or nonsilencing control lentiviruses, stimulated for 10 s with 40 mM K⁺ and immediately fixed. Data from two independent experiments done in duplicate (10–15 neurons each). Error bars represent SEM.