Figure 7.

Knockdown of α and βCaMKII inhibits L-type channel signaling to CREB. (A) pCREB levels from neurons stimulated for 2.5 s with 5- or 40-mM K⁺ Tyrode's and transferred to a 5 mM K⁺ solution for 45 s before fixation. Cultures were infected with GFP alone, α and βCaMKII, or nonsilencing lentiviruses. Data are from three to six independent experiments (15–30 cells from duplicate coverslips in each). *, P < 5 × 10⁻¹⁷; **, P < 5 × 10⁻¹⁰. (B) Total nuclear CREB levels in neurons infected with GFP alone or α and βCaMKII lentiviruses. Data from three independent experiments, two done in parallel with pCREB experiments in A (46–87 neurons). (C) Peak Ca²⁺ elevations (Δ[Ca²⁺]_i) from sister cultures to those in B. Data are from seven separate experiments (31–49 cells). Error bars represent SEM.