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. 2008 Dec 1;183(5):769–776. doi: 10.1083/jcb.200808097

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© 2008 Mortusewicz et al.

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Figure 1. — PC4 accumulates at DNA damage sites. HeLa cells were treated with 10 mM HU, 10 mM H₂O₂, or 10 μg/ml Aph for the indicated time points and in situ extracted with 0.5% Triton X-100 for 30 s before fixation. (A and B) Widefield fluorescence images of human HeLa cells treated with H₂O₂ or HU show accumulation of PC4 at subnuclear sites colocalizing with the DNA damage markers PAR and γH2AX, respectively. (C) Replication arrest with HU or Aph, as well as DNA damage induction with H₂O₂ results in relocalization of PC4 to subnuclear foci colocalizing with the single strand binding protein RPA34. Bars, 5 μm.