TABLE 1.
Comparison of ratios of gene expression for A. fumigatus alone and A. fumigatus treated with AMB, obtained with microarray hybridization and real-time RT-PCR
| Locus tag | Protein description | Fold increasea
|
|
|---|---|---|---|
| Microarray hybridization | Real-time RT-PCR | ||
| Afu7g03740 | 14-Alpha demethylase Cyp51A (ERG11) | 2.8 | 2.0 |
| Afu4g03630 | Sterol 24-C-methyltransferase (ERG6) | −2.9 | −0.76 |
| Afu6g07820 | Integral membrane protein, putative | 2.6 | 2.0 |
| Afu1g14550 | Mn-SOD | 2.3 | 3.1 |
| Afu3g07930 | Putative GST | 3.2 | 2.3 |
| Afu5g11380 | Rho-GDP dissociation inhibitor | 2.0 | 5.2 |
| Afu2g11150 | Secretory-pathway GDI | 2.0 | 4.0 |
| Afu6g12340 | GTPase-activating protein, putative | 2.5 | 4.5 |
| Afu7g04580 | TBC domain protein, putative | 2.0 | 1.9 |
| Afu3g03500 | Multidrug resistance protein 4, putative | 3.5 | 2.1 |
| Afu3g07640 | Plasma membrane H+ ATPase | 3.4 | 5.4 |
| Afu3g13670 | Siderochrome-iron transporter, putative | 4.4 | 1.9 |
a
Values shown are ratios for A. fumigatus treated with AMB for 24 h (at the MIC50) versus A. fumigatus cultured alone for 24 h. Changes in expression levels of target genes are averages for replicates, normalized to GAPDH mRNA expression.