TABLE 2.

Oligonucleotide primers

Procedure(s) and target gene	Primer name	Sequencea	Amplicon size (bp)
PCR and sequencing
pbp1a PCR	f1	5′-CGGAATTCATGGGATTTATTATCTTAGCTA-3′	2,209
	r1	5′-ACGTCGACTTAATTACCGTTAGGTACTGTA-3′
pbp1a sequencing primers	f1b	5′-ACACCAAAGAAGAAATTCTTAC-3′
	f2	5′-TAAAGCAAAAATCTACTTATCC-3′
	f2b	5′-GTAGTGAGAAAATGGCAGCGGC-3′
	f3	5′-GCCTACATGATGACGGATATGC-3′
	f3b	5′-CAAAATTCTGGACAGTCAAGTC-3′
	r2	5′-TCCAATCTGCACTGTATCCGCC-3′
	r3	5′-TAGCTGCTTTAGTACCAGTACC-3′
	r4	5′-CAGCGGCTTCAAGTGCTCTGAC-3′
	r5	5′-TGACTTTACCATTAGTCGCATC-3′
	r6	5′-TTTTATCTTGATACATCTGCTG-3′
pbp1b PCR	f1	5′-CGGAATTCATGTTTAAAGGTAATAAGAAGT-3′	2,314
	r1	5′-ACGTCGACTTATCGTTTTCCACCCAAAGTA-3′
pbp1b sequencing primers	f1b	5′-GGTTTGGAGAGAGTAGCGG-3′
	f2	5′-CTATTGTATATTCTCCTTATAC-3′
	f2b	5′-GTATACTATTAAAACTACTATC-3′
	f3	5′-TGATGTAAAAAACTATATGGAG-3′
	f3b	5′-CCTGTCCGTGTCTTTTCGAAAG-3′
	r2	5′-GTGTAGAAAGCATCAACCAAAC-3′
	r3	5′-GAGCAACTGACGTATCAATACC-3′
	r4	5′-GATCAATAGCAATTCCGTAAGG-3′
	r5	5′-TTTTTAAATCATGCTCTGAAAC-3′
	r6	5′-GTAAACCTGCAAGGAAAGCTGC-3′
pbp2a PCR	f1	5′-CGGGATCCATGAAATTATTTGATAAGTTTA-3′	2,338
	r1	5′-ACGTCGACCTATCTAAAGTAGTCCTTTAGA-3′
pbp2a sequencing primers	f1b	5′-TGCTCTAAAAACAACCACCACC-3′
	f2	5′-ATCTTAATAACTCTTATTTTGG-3′
	f2b	5′-GGTATGAAAAATAGATTAGCAG-3′
	f3	5′-TCCTGCTGTTTATACTTTAGAC-3′
	f3b	5′-ACTCGAATTGAGACAGCTAATG-3′
	r2	5′-CTGTCAAATAATGGTGTTTATC-3′
	r3	5′-ATGAGCGCGATGCATTATACCG-3′
	r4	5′-GTTCTTTATCTATTGACCATCC-3′
	r5	5′-TATAGCCATTATTGACAATATC-3′
	r6	5′-TCAAATTAGCAGCACTGGTTCC-3′
pbp2b PCR	f1	5′-CGGAATTCATGTTGAATCGTAAAAAAAGGT-3′	2,062
	r1	5′-ACGTCGACTTATTGTCCTGTGAACTGTGAA-3′
pbp2b sequencing primers	f1b	5′-TTCATCTCAGTCTATCAAAGAG-3′
	f2	5′-CAACTCTAATGGAATCGTTCGG-3′
	f2b	5′-CTATTTCTACAGAAAAGGCAGG-3′
	f3	5′-AGAAAGTATCTTGAAACAATAC-3′
	f3b	5′-TGGACAAACAGTTTCTACCTAC-3′
	r2	5′-CTATCTTATTTAGTGTTTTAGG-3′
	r3	5′-GATAGCCTCGATCAGTTAAAGC-3′
	r4	5′-CATGATCATTTTTCAGACCAGC-3′
	r5	5′-CTCGGTCATTCAGTGAATAGCC-3′
	r6	5′-TAGCGCTCACTGGAACTGCAGC-3′
pbp2x PCR	f1	5′-CGGAATTCGTGACTTTTTTTAAAAAGCTAA-3′	2,275
	r1	5′-ACGTCGACTTAATCTCCTATTGTAATTTTG-3′
pbp2x sequencing primers	f1b	5′-AACTATACGACAGCTACAGGTC-3′
	f2	5′-GTAGTGGGAATGTTCTTTTAGG-3′
	f2b	5′-TCTAAGCATTTTAACTCTACTG-3′
	f3	5′-AAGAAGCAGCTAGTAAAACACG-3′
	f3b	5′-GAAAATCCAGGTCATGTAGCGG-3′
	r2	5′-GAACCAGATTACGACGTAATTC-3′
	r3	5′-CAGATTTTACTGCAACTGATTG-3′
	r4	5′-ATGAGCTCATAGCGATAGTTAC-3′
	r5	5′-TTGCAGAGGCTAGAGTCATTAC-3′
	r6	5′-CCGCCCTACGTTCTGTTGTTGC-3′
	r7	5′-AAGACAATCCTGAACCTGAACTTCC-3′
	r8	5′-TATCTGTACCAACGATGATGAC-3′
Real-time RT-PCR
pbp2x	f1	5′-ACCGGGATTGAATGACTCAG-3′	109
	r1	5′-TGGCTGAACCAGATTACGAC-3′
pros	f1	5′-AATGCCAAGTGATGCTCAGG-3′	84
	r1	5′-GCATAGATTCCAGCCGAAAC-3′

^aRestriction sites are underlined.