FIG. 6.

Stable cells containing the replicons obtained from the cells selected with PSI-6130 show moderate reduced sensitivity to PSI-6130 compared to NM107 results. Whole cellular RNA was isolated from the cells selected with PSI-6130 and transfected into naïve Huh-7 cells. Stable cell lines were generated from selection set 2 (5R32) and from untreated cells (RC57). The generated stable cell lines were tested for sensitivity to PSI-6130, NM107, and NNI-1 by the use of qPCR (error bars represent the standard errors of the means of the results of three independent experiments). The shift values were calculated based on the untreated cell line value, which was set to 1.