TABLE 4.
PSI-6130 activity against GT-1b clinical isolates with the S282T substitution
| Strain and mutationa | % Replication capacityb | EC50 (μM [mean ± SEMc])
|
|
|---|---|---|---|
| PSI-6130 | NM107 | ||
| Con1 | |||
| None | 100 | 0.82 ± 0.04 | 0.52 ± 0.07 |
| S282T | 15 | 2.51 ± 0.29 | >30d |
| RO-1-H06 | |||
| None | 100 | 0.28 ± 0.04 | 0.60 ± 0.13 |
| S282T | 15 | 1.07 ± 0.35 | >30d |
| RO-2-D09 | |||
| None | 100 | 0.25 ± 0.02 | 0.66 ± 0.06 |
| S282T | 26 | 0.90 ± 0.095 | >30d |
| RO-3-D10 | |||
| None | 100 | 0.26 ± 0.03 | 0.66 ± 0.11 |
| S282T | 6 | 1.41 ± 0.24 | >30d |
| RO-11-D07 | |||
| None | 100 | 0.30 ± 0.03 | 0.52 ± 0.06 |
| S282T | 25 | 1.04 ± 0.17 | >30d |
a
RO-1-H06, RO-2-D09, RO-3-D10, and RO-11-D07 are single molecular clones representing the consensus sequences of RO-1, RO-2, RO-3 (Fig. 2), and RO-11 (data not shown), respectively.
b
The replication capacity for each S282T mutant is expressed as the percentage of the corresponding parent sequence.
c
SEM values represent standard errors of the means of the results of a minimum of three independent experiments.
d
Maximum concentration of NM107 used in the assay.