FIG. 4.
Cellular potency. (A) Average percent reduction in HCV replicon RNA in Huh7 cells promoted by the indicated concentrations of ITMN-191 as determined by RT-PCR from 18 independent experiments. The standard deviation between experiments is indicated. (B) HCV replicon RNA copies per cell as determined by RT-PCR during a 2-week treatment in the absence or presence of the indicated concentrations of ITMN-191 (shaded gray) and 4-week follow-up. Averaged data from two independent experiments are shown. The limit of detection by RT-PCR was 4.5 × 10−3 ± 1.9 × 10−4 copies/cell. (C) Number of cells per culture well during a 2-week treatment in the absence or presence of the indicated concentrations of ITMN-191 (shaded gray) and 4-week follow up. Averaged data from two independent experiments are shown.