FIG. 6.

Bactericidal effect of EIP-K and H₂O₂ presented in different temporal combinations. EIP-K (27.5 mM) and H₂O₂ (5 mM) were presented to bacteria at pH 7 singly for 10 min, simultaneously for 10 min (EIP-K + H₂O₂), sequentially with EIP-K first for 10 min followed by rinsing and then by H₂O₂ for 10 min (EIP-K→H₂O₂), sequentially with H₂O₂ first for 10 min followed by rinsing with LB then by EIP-K for 10 min (H₂O₂→EIP-K), or with neither EIP-K nor H₂O₂ (Control). Only EIP-K + H₂O₂ produced significant and substantial bactericidal activity. Values are means ± standard errors of the means (two to four experiments, each run in triplicate). An asterisk indicates that the value for the product with H₂O₂ is significantly lower than that for the product without H₂O₂ (P < 0.05; paired t test).