Phylogenetic affiliations of 16S rRNA gene sequences obtained by 13C-labeled SIP incubations with methanol (MOH), monomethylamine (MMA), dimethylamine (DMA), methyl bromide (MBr), and DMS SIP incubations. Selected GenBank sequences from uncultivated clones and reference strains are included for comparison. Bootstrap values are included for all branch points on this neighbor-joining tree. GenBank accession numbers are shown in parentheses. The scale bar (in the tree) represents 5% sequence divergence. The collapsed clades are expanded in Fig. S2, S3, and S4 in the supplemental material. The division-level affiliation of sequences is indicated in the boxes at the right. CFB, Cytophaga-Flavobacterium-Bacteroides.