FIG. 1.

(a) Distribution of the soluble version of the aggregation-prone mGFP (triangles) and fluorescence emission (circles) along a sucrose gradient. (b) Specific fluorescence emission of soluble GFP populations. Arrows indicate discrete peaks of highly fluorescent protein subfractions (with GFP purity values given as percentages), and the inset shows values for the average specific fluorescence of soluble GFP in the whole soluble cell fraction. (c) FTIR spectra (normalized at the tyrosine peak by use of GRAMS/AI spectroscopic software, version 7) of isolated GFP inclusion bodies (dashed line) and the purified GFP population (from peak 1) (continuous line). Vertical lines indicate the relevant peaks corresponding to α-helix (α), unfolded stretches (U), native intramolecular β-sheet (β intra), and intermolecular cross β-sheet (β inter).