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Transcriptional analysis of kshA2, kstD1 (positive control for AD induction), and 16S rRNA (control) by RT-PCR using total RNA isolated from noninduced cultures (lane 1) and AD-induced cultures of parent strain R. erythropolis SQ1 (lane 2). PCR primers used are RT-KSHA2-F and RT-KSHA2-R for kshA2, RT-KSTD1-F and RT-KSTD1-R for kstD1, and 16SRNA-RHODO-F and 16SRNA-RHODO-R for 16S rRNA (Table 1).
