FIG. 1.

Fragment of lacZ-tcdA1B1 which is present in the pET-32a(+)-tcdA1B1 (with SphI and BamHI sites in the vector) and pMini-Tn5 (with NotI sites) constructs expressing tcdA1B1 genes. The tcdA1B1 fragment includes digested DNA containing a 7,255-bp downstream sequence of tcdA1, a 4,431-bp sequence of tcdB1, and a partial sequence (2,488 bp) of tccC2 obtained using KpnI and BamHI, as well as the amplified 376-bp upstream sequence of tcdA1, from P. luminescens TT01 genomic DNA. The enzyme sites indicated are the sites used for subcloning (see text). Sizes are also indicated.