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. 2008 Oct 3;74(23):7219–7226. doi: 10.1128/AEM.00977-08

FIG. 2.

FIG. 2.

SDS-PAGE analysis (8% gel) of TcdA1B1 proteins (A) and Western blot analysis of the same samples with TcdA1B1 polyclonal antisera (B). Lane M1, TaKaRa narrow-range protein molecular mass standards (44.3 to 200 kDa); lane M2, Hou-Bio prestained SDS-PAGE standards (2 to 200 kDa); lane 1, E. cloacae cells carrying a transposon with the tcdA1B1 genes; lane 2, E. cloacae cells carrying a transposon without the tcdA1B1 genes; lane 3, E. cloacae cells lacking a transposon; lane 4, P. luminescens TT01 cells; lane 5, recombinant TcdA1B1 proteins extracted from an E. coli BL21(DE3)/pET-32a(+)-tcdA1B1 culture induced with IPTG; lane 6, E. coli BL21(DE3)/pET-32a(+)-tcdA1B1 culture induced with IPTG.